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KMID : 0371620010160010013
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Journal of Wonkwang Medical Science
2001 Volume.16 No. 1 p.13 ~ p.24
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Effect of Protein kinase C inhibitor on mesangial cells in High Glucose
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Jang Geun-Young
Park Byoung-Hyun
Park Seung-Taeck
Cho Chung-Gu
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Abstract
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Hyperglycemia increases the level of diacylglycerol(DAG) and activates protein kinase C(PKC) in mesangial cells and other vascular tissues. Growth factors, especially TGF-β, play important roles in mesangial cell proliferation and in pathophysiology of diabetic nephropathy.In order to clarify the interaction between PKC and TGF-β in high glucose condition, high glucose condition was induced by the application of glucose to medium containing mesangial cells derived from neonatal mouse. Mesangial cells were incubated with various concentrations of glucose for 60 hours. The effects of high glucose, TGF-β and arachidonic acid as a PKC activator on cell proliferation were examined. In addition, the effect of calphostin C, an inhibitor of PKC, on TGF-β induced cell proliferation in high glucose condition was examined in these cultures. After incubation with various concentrations of glucose for 60 hours, the number of cultured mouse mesangial cells were decreased in a dose-dependent manner and LC_50 of glucose was 20mM. Both arachidonic acid and TGF-β increased mesangial cell proliferation dose-dependently. But, mesangial cells treated with TGF-β and calphostin C were markedly decreased compaired with TGF-β only treated. These results suggest that high glucose concentration induce toxic effect in proliferation of cultured mouse mesangial cells and TGF-β may induce cell proliferation via activation of PKC in high glucose condition.
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KEYWORD
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Mesangial cell, Protein Kinase C
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